Validating the assessment of glucose 6 phosphate dehydrogenase g6pd
Cytochemical assays can obviate these shortcomings, but at the expense of added technical complexity and labor.
We describe here a simple, novel cytofluorometric method that extends the classic methemoglobin reduction test, assessing G6PD deficiency at the level of an individual erythrocyte.
However, there is no suitable animal model that can predict the clinical hemolytic potential of drugs.
We developed and validated a human (hu)RBC-SCID mouse model by giving nonobese diabetic/SCID mice daily transfusions of hu RBCs from G6PD-deficient donors.
Limited studies have investigated the incidence of G6PD mutations and their functional role in causing disease among Arab countries. Further, using “genion” tool, the system was neutralised by adding Chlorine ions as there was an overall positive charge.